Anticorps Recombinant de lapin anti-iNOS

iNOS Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

6O22

N° de cat : 80517-1-PBS

Synonymes

NOS2, Inducible NOS, Inducible NO synthase, HEP-NOS, Hepatocyte NOS

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Galerie de données de validation

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  WB analysis using 80517-1-RR (same clone as 80517-1-PBS)

  Untreated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  WB analysis of human PBMCs using 80517-1-RR (same clone as 80517-1-PBS)

  LPS treated human PBMCs were subjected to SDS PAGE followed by western blot with 80517-1-RR (iNOS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 80517-1-RR (same clone as 80517-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 80517-1-RR (iNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80517-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80517-1-PBS cible iNOS dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nitric oxide synthase 2, inducible
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 110-130 kDa
• Numéro d’acquisition GenBank: NM_000625
• Symbole du gène: iNOS
• Identification du gène (NCBI): 4843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

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