Anticorps Polyclonal de lapin anti-iNOS

• Phare
• Validé par KD/KO

iNOS Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

2

N° de cat : CL594-18985

Synonymes

HEP NOS, Hepatocyte NOS, Inducible NO synthase, Inducible NOS, INOS, NOS, NOS type II, NOS2, NOS2A

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Galerie de données de validation

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  IF Staining of HeLa using CL594-18985

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 iNOS antibody (CL594-18985) at dilution of 1:100. Blue (DAPI).

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  IF Staining of HepG2 using CL594-18985

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 iNOS antibody (CL594-18985) at dilution of 1:100. Blue (DAPI)

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  FC experiment of HepG2 using CL594-18985

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human iNOS (CL594-18985) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL594-18985 cible iNOS dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nitric oxide synthase 2, inducible
• Masse moléculaire calculée: 131 kDa
• Poids moléculaire observé: 110-130 kDa, 65-70 kDa
• Numéro d’acquisition GenBank: NM_000625
• Symbole du gène: NOS2
• Identification du gène (NCBI): 4843
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NOS2, also named as iNOS and NOS2A, produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission, antimicrobial and antitumoral activities. NOS2 is a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. iNOS has a very short half-life due to rapid degradation by calpain. iNOS monomer is a direct substrate of calpain I and can be cleaved by calpain I at the canonical CaM-binding site(503-532aa) of iNOS, and then a ~70kD band can be detected by western (PMID:11786228). This antibody is specific to NOS2.

Publications

Species	Application	Title
mouse	IF	J Ethnopharmacol; Elsholtzia bodinieri Vaniot ameliorated acute lung injury in mice by regulating pyroptosis, inflammation, oxidative stress and macrophage polarization; Authors - Xiaoqian Jiang
mouse	IF	Biomaterials; Magnesium/gallic acid bioMOFs laden carbonized mushroom aerogel effectively heals biofilm-infected skin wounds; Authors - Ganghua Yang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C