Anticorps Polyclonal de lapin anti-IL-33

IL-33 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 28035-1-AP

Synonymes

Il33, Il 33, Il1f11, interleukin 33, NF HEV

---

Galerie de données de validation

• 

  WB analysis of mouse lung using 28035-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 28035-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 28035-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 28035-1-AP (il33 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse spleen using 28035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28035-1-AP (IL-33 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 28035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28035-1-AP (IL-33 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 28035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28035-1-AP (IL-33 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, tissu de côlon de souris, tissu pulmonaire de rat
• Résultats positifs en IHC: tissu splénique de souris, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28035-1-AP cible IL-33 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-33 Protéine recombinante Ag27846
• Nom complet: interleukin 33
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: NM_001164724
• Symbole du gène: IL-33
• Identification du gène (NCBI): 77125
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-33 (IL-33) is a tissue-derived nuclear cytokine from the IL-1 family abundantly expressed in endothelial cells, epithelial cells and fibroblast-like cells, both during homeostasis and inflammation. IL-33 activates many immune cell types involved in type-2 immunity and allergic inflammation, including ILC2s, mast cells, Th2 cells, eosinophils, basophils, dendritic cells and alternatively activated macrophages (AAM). As a cytokine, IL-33 interacts with the receptors ST2 (also known as IL1RL1) and IL-1 Receptor Accessory Protein (IL1RAP), activating intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. IL-33 is also effective in reversing Alzheimer-like symptoms in APP/PS1 mice, by reversing the buildup and preventing the new formation of amyloid plaques. IL-33 is synthesized as a 30-34 kD full-length form and 20 kDa form mature form.

{{ptg:RelatedPrimaryAntibodies}}