Anticorps Polyclonal de lapin anti-Ki-67

Ki-67 Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

N° de cat : CL647-28074

Synonymes

Mki67, ki67, Ki 67, Proliferation related Ki 67 antigen

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Galerie de données de validation

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  IF Staining of mouse colon using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  IF Staining of mouse colon using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  IF Staining of HeLa using CL647-28074

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 ki67 antibody (CL647-28074) at dilution of 1:200.

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  FC experiment of Jurkat using CL647-28074

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of NIH/3T3 using CL647-28074

  1x10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated Ki-67 Polyclonal antibody (CL647-28074)(red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF-P: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules NIH/3T3, cellules Jurkat

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-28074 cible Ki-67 dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Ki-67 Protéine recombinante Ag27894
• Nom complet: antigen identified by monoclonal antibody Ki 67
• Masse moléculaire calculée: 351 kDa
• Numéro d’acquisition GenBank: NM_001081117
• Symbole du gène: ki67
• Identification du gène (NCBI): 17345
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry.

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