Anticorps Polyclonal de lapin anti-mCherry

mCherry Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26765-1-PBS

Synonymes

Red fluorescence protein, red fluorescent protein, RFP, RFP-tag

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Galerie de données de validation

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  WB analysis using 26765-1-AP (same clone as 26765-1-PBS)

  mCherry tagged protein was subjected to SDS PAGE followed by western blot with 26765-1-AP (mCherry antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  WB result of mCherry antibody (26765-1-AP; 1:2000; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  WB analysis of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  WB result of mCherry antibody (26765-1-AP; 1:300; room temperature for 1.5 hours) with negative control and mCherry overexpressed Transfected HEK-293 cells. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  IP experiment of Transfected HEK-293T using 26765-1-AP (same clone as 26765-1-PBS)

  IP result of anti-mCherry (IP:26765-1-AP, 4ug; Detection:26765-1-AP 1:2000) with Transfected HEK-293T cells lysate 1280 ug. This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 26765-1-AP (same clone as 26765-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (26765-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26765-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26765-1-PBS cible mCherry dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: mCherry Protéine recombinante Ag25320
• Nom complet: mCherry
• Masse moléculaire calculée: 27 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Red fluorescent proteins (RFPs) is a collective term referring to a heterogenous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages.

The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm).

When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody raised against mCherry. It can be used to detect mCherry, dsRed, tdTomato, and mScarlet.

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