Anticorps Monoclonal anti-mCherry
mCherry Monoclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Protéine recombinante et plus (2)
Applications
WB, IF/ICC, IP, CoIP, ELISA
Conjugaison
Non conjugué
CloneNo.
1B3C6
N° de cat : 68088-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293 transfectées |
| Résultats positifs en IF/ICC | cellules HEK-293 transfectées, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Informations sur le produit
68088-1-Ig cible mCherry dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, porc |
| Hôte / Isotype | Mouse / IgG1 |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | mCherry Protéine recombinante Ag25320 |
| Nom complet | mCherry |
| Masse moléculaire calculée | 27 kDa |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine G |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for mCherry antibody 68088-1-Ig | Download protocol |
| IF protocol for mCherry antibody 68088-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Open Biol Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21 | ||
J Virol Swine RNF5 positively regulates the antiviral activity of IFITM1 by mediating the degradation of ABHD16A | ||
J Biol Chem Porcine IKKε is involved in the STING-induced type I IFN antiviral response of the cytosolic DNA signaling pathway |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Olga (Verified Customer) (04-09-2025) | Very clear and strong signal in Western blot.
 |
FH Christine (Verified Customer) (10-02-2024) | We are running out of a home-made sheep anti RFP antibody and trying to find a replacement. I ran samples transfected with a mCHERRY-tagged protein side by side to compare our old antibody and this one. Although I detected clearly the expression of the mCHERRY tagged protein with this antibody, it was not as sensitive as the sheep one we had and there were some bands of my protein of interest that I could not detect clearly. Upping the concentration from 1:5,000 to 1:1,000 did not make any clear difference. So although the antibody works it is not sensitive enough for my needs.
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