Anticorps Monoclonal anti-mCherry

mCherry Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B3C6

N° de cat : 68088-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

  Non-transfected and transfected HEK-293 lysates were subjected to SDS PAGE followed by western blot with 68088-1-Ig (mCherry antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

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  IF Staining of Transfected HEK-293 using 68088-1-Ig (same clone as 68088-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using mCherry antibody (68088-1-Ig, Clone: 1B3C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68088-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68088-1-PBS cible mCherry dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: mCherry Protéine recombinante Ag25320
• Nom complet: mCherry
• Masse moléculaire calculée: 27 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

mCherry is a fluorophore (a fluorescent protein) used in biotechnology as a tracer to follow the flow of fluids, as a marker when tagged to molecules and cell components. mCherry is the second generation monomeric red fluorescent protein that have improved brightness and photostability. mCherry and the majority of red fluorescent proteins derive from a protein isolated from Discosoma sp. mCherry is a monomeric fluorescent construct with peak absorption/emission at 587 nm and 610 nm, respectively.

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