Anticorps Recombinant de lapin anti-mCherry

mCherry Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241830F8

Avis (1)

N° de cat : 81202-2-RR

Synonymes

241830F8

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Galerie de données de validation

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  WB analysis using 81202-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81202-2-RR (mCherry antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of Transfected HEK-293 using 81202-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using mCherry antibody (81202-2-RR, Clone: 241830F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of Transfected HEK-293T using 81202-2-RR

  1x10^6 HEK-293T cells or mCherry Transfected HEK-293T cells were intracellularly stained with 0.25 ug mCherry Recombinant antibody (81202-2-RR, Clone:241830F8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81202-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81202-2-RR against mCherry were performed. The affinity constant is 0.205 nM.

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Applications testées

• Résultats positifs en WB: Transfected HEK-293T cells,
• Résultats positifs en IF/ICC: cellules HEK-293 transfectées,
• Résultats positifs en FC (Intra): Transfected HEK-293T cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81202-2-RR cible mCherry dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: mCherry Protéine recombinante Ag25320
• Nom complet: mCherry
• Masse moléculaire calculée: 27 kDa
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Red fluorescent proteins (RFPs) is a collective term referring to a heterogeneous group of red chromophore-carrying proteins, originating from various species and forming different protein lineages. The original RFP (dsRed) is a 225 amino acid fluorescent protein (25.9 kDa) derived from Discosoma sp.. It emits red light with a peak wavelength of 593 nm upon excitation by green light (excitation peak at 558 nm). When fused with other proteins, RFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitating visualization of subcellular localization through fluorescence microscopy. This antibody is a rabbit polyclonal antibody raised against mCherry.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (09-23-2025)

Excellent, strong and very clean antibody recognising mCHERRY tagged proteins overexpressed in HEK293T cells. No background at all.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: Transfected HEK293T

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