Anticorps Recombinant de lapin anti-mDia1

mDia1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

243001A5

N° de cat : 85708-2-RR

Synonymes

DIAPH1, DIAP1, DIAPH 1, Diaphanous related formin 1, Diaphanous-related formin-1

---

Galerie de données de validation

• 

  WB analysis using 85708-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85708-2-RR (mDia1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 85708-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using mDia1 antibody (85708-2-RR, Clone: 243001A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 85708-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85708-2-RR against Human mDia1 were performed. The affinity constant is 3.88 nM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MDA-MB-231, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85708-2-RR cible mDia1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: mDia1 Protéine recombinante Ag14523
• Nom complet: diaphanous homolog 1 (Drosophila)
• Masse moléculaire calculée: 1272 aa, 141 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC007411
• Symbole du gène: mDia1
• Identification du gène (NCBI): 1729
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mDia1, also known as DIAPH1 or Diap1, is a mammalian diaphanous-related formin which is implicated in multiple physical and pathological events including cytoskeletal dynamics, autosomal hearing loss, and myelodysplasia. Depending upon the cell type and position in the cell cycle, mDia1 has been shown to localize to the cell cortex, trafficking endosomes, cleavage furrow, mid-bodies, and centrosomes, the cytoplasmic microtubule-organizing center crucial for cell division. Mutation of mDia1 has been linked to microcephaly. This antibody recognizes the endogenous mDia1 mainly around 140-150 kDa, while sometimes an additional 70 kDa can also be observed which is proposed to be a fragment of 140-150 kDa molecules (26011179).

{{ptg:RelatedPrimaryAntibodies}}