Anticorps Polyclonal de lapin anti-mNeonGreen

mNeonGreen Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Protéine recombinante et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 29523-1-AP

Synonymes

mNeon Green, blFP-Y3

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Galerie de données de validation

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  WB analysis using 29523-1-AP

  mNeonGreen tagged proteins and Native HEK293 cell lysates were subjected to SDS PAGE followed by western blot with 29523-1-AP (mNeonGreen antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

29523-1-AP cible mNeonGreen dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Protéine recombinante

• Réactivité: Protéine recombinante
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: mNeonGreen Protéine recombinante Ag31617
• Nom complet: mNeonGreen
• Masse moléculaire calculée: 29 kDa
• Numéro d’acquisition GenBank: PF01353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

mNeonGreen is a basic (constitutively fluorescent) green/yellow fluorescent protein published in 2013, derived from Branchiostoma lanceolatum. It is reported to be a very rapidly-maturing monomer with moderate acid sensitivity. This antibody can be used to detect the mNeonGreen tagged recombinant protein.

Publications

Species	Application	Title
human	WB	Proc Natl Acad Sci U S A; Unlicensed origin DNA melting by MCV and SV40 polyomavirus LT proteins is independent of ATP-dependent helicase activity; Authors - Li Wan
	WB	Elife; A Tad-like apparatus is required for contact-dependent prey killing in predatory social bacteria; Authors - Sofiene Seef
human	WB,IF	Cancer Cell Int; Regulation of microtubule nucleation in glioblastoma cells by ARF GTPase-activating proteins GIT1 and GIT2 and protein kinase C; Authors - Vadym Sulimenko

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (03-18-2024)

Not very clean, some non-specific bands, mNG-tagged protein can be observed. HEK293T lysates 25ug.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

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