Anticorps Monoclonal anti-Nectin-4/PVRL4

Nectin-4/PVRL4 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

2D4B6

N° de cat : 67721-1-Ig

Synonymes

Ig superfamily receptor LNIR, LNIR, nectin 4, Nectin-4, poliovirus receptor related 4, PRR4, PVRL4

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Galerie de données de validation

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  WB analysis of human placenta using 67721-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 67721-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 67721-1-Ig (Nectin-4/PVRL4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

67721-1-Ig cible Nectin-4/PVRL4 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Nectin-4/PVRL4 Protéine recombinante Ag14414
• Nom complet: poliovirus receptor-related 4
• Masse moléculaire calculée: 510 aa, 55 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC010423
• Symbole du gène: Nectin-4
• Identification du gène (NCBI): 81607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nectin-4, also known as PVRL4, is a type-I transmembrane glycoprotein that belongs to the nectin subfamily of immunoglobulin-like adhesion molecules that participate in Ca(2+)-independent cell-cell adhesion (PMID: 18703497). The extracellular domain of Nectin-4, which contains three immunoglobulin-like domains (V and two C2-type domains, VCC), can be proteolytically cleaved to release a soluble form. Nectin-4 interacts with afadin via its carboxyl-terminal cytoplasmic sequence and trans-interacts with nectin-1/PRR1 through V domain interaction (PMID: 11544254). It acts as a receptor for measles virus (PMID: 22048310). Nectin-4 is overexpressed in multiple human malignancies and the aberrant expression is correlated with cancer progression and poor prognostic (PMID: 35131876). Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

Publications

Species	Application	Title
human	WB,IHC	Cell Rep; High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy; Authors - Zhuo Xing

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