Anticorps Recombinant de lapin anti-Nectin-4/PVRL4

Nectin-4/PVRL4 Recombinant Antibody for IF/ICC, FC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240009F2

N° de cat : 83223-4-PBS

Synonymes

Nectin-4, PVRL4, 240009F2, Ig superfamily receptor LNIR, LNIR

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Galerie de données de validation

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  IF Staining of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Nectin-4/PVRL4 antibody (83223-4-RR, Clone: 240009F2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83223-4-RR (same clone as 83223-4-PBS)

  1x10^6 MCF-7 cells were surface stained with 0.25 ug Nectin-4/PVRL4 Recombinant Antibody (83223-4-RR, Clone: 240009F2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83223-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83223-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83223-4-RR against Human Nectin-4/PVRL4 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83223-4-PBS cible Nectin-4/PVRL4 dans les applications de IF/ICC, FC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Nectin-4/PVRL4 Protéine recombinante Eg0415
• Nom complet: poliovirus receptor-related 4
• Masse moléculaire calculée: 510 aa, 55 kDa
• Numéro d’acquisition GenBank: BC010423
• Symbole du gène: Nectin-4
• Identification du gène (NCBI): 81607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625).

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