Anticorps Recombinant de lapin anti-nectin 4

nectin 4 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240009B8

N° de cat : 83223-7-PBS

Synonymes

Nectin-4, PVRL4, poliovirus receptor related 4, Nectin cell adhesion molecule 4, LNIR

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00147-4

  Sandwich ELISA standard curve of MP00147-4, Human Nectin-4/PVRL4 Recombinant Matched Antibody Pair - PBS only. 83223-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0415. 83223-7-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/ml.

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  Sample test of MP00147-4

  Serum of sixteen individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 750.17 pg/mL with a range of 392.48 - 1,810.23 pg/mL.

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  Sample test of MP00147-4

  Urine of eight individual healthy human donors was measured. The Nectin-4/PVRL4 concentration of detected samples was determined to be 3,920.62 pg/mL with a range of 249.12 - 9,633.39 pg/mL.

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  Sample test of MP00147-4

  MCF-7 human breast cancer cells (5 x 10^6 cells/mL) were cultured in DMEM and 10% fetal bovine serum, 4 mM L-glutamine, 4500 mg/L glucose, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. 1 x 10^6 cells/mL were lysated by RIPA buffer, and the supernate was assayed and measured 3.2 ng/mL.

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  Sample test of MP00147-4

  The mean Nectin-4/PVRL4 concentration was determined to be 102.1 ng/mL in MDA-MB-468 cell extract based on a 3.1 mg/mL extract load and 107.4 ng/mL in MCF-7 cell extract based on a 4.2 mg/mL extract load.

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Informations sur le produit

83223-7-PBS cible nectin 4 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: poliovirus receptor-related 4
• Masse moléculaire calculée: 510 aa, 55 kDa
• Numéro d’acquisition GenBank: BC010423
• Symbole du gène: Nectin-4
• Identification du gène (NCBI): 81607
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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