Anticorps Monoclonal anti-p120 Catenin

• Phare
• Validé par KD/KO

p120 Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F7H8

N° de cat : 66208-1-PBS

Synonymes

CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1

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Galerie de données de validation

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  WB analysis of fetal human brain using 66208-1-Ig (same clone as 66208-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66208-1-Ig (same clone as 66208-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66208-1-Ig (same clone as 66208-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66208-1-Ig (same clone as 66208-1-PBS)

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66208-1-Ig (same clone as 66208-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66208-1-Ig(p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66208-1-PBS cible p120 Catenin dans les applications de WB, IHC, IF/ICC, IF-Fro, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p120 Catenin Protéine recombinante Ag2824
• Nom complet: catenin (cadherin-associated protein), delta 1
• Masse moléculaire calculée: 948 aa, 105 kDa
• Poids moléculaire observé: 90-120 kDa
• Numéro d’acquisition GenBank: BC010501
• Symbole du gène: p120 Catenin
• Identification du gène (NCBI): 1500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

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