Anticorps Monoclonal anti-p130Cas / BCAR1

p130Cas / BCAR1 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D11C10

N° de cat : 67215-1-PBS

Synonymes

BCAR1, 2D11C10, CASS1, CAS1, CAS

---

Galerie de données de validation

• 

  WB analysis using 67215-1-Ig (same clone as 67215-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67215-1-Ig (p130Cas / BCAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using p130Cas / BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human breast cancer using 67215-1-Ig (same clone as 67215-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using BCAR1 antibody (67215-1-Ig, Clone: 2D11C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 67215-1-Ig (same clone as 67215-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug p130Cas / BCAR1 Monoclonal antibody (67215-1-Ig, Clone:2D11C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67215-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67215-1-PBS cible p130Cas / BCAR1 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p130Cas / BCAR1 Protéine recombinante Ag10604
• Nom complet: breast cancer anti-estrogen resistance 1
• Masse moléculaire calculée: 870 aa, 93 kDa
• Poids moléculaire observé: 116 kDa
• Numéro d’acquisition GenBank: BC062556
• Symbole du gène: BCAR1
• Identification du gène (NCBI): 9564
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}