Anticorps Polyclonal de lapin anti-Cdkn2a/p16INK4a

Cdkn2a/p16INK4a Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 28416-1-AP

Synonymes

Cdkn2a, p16INK4a, Cyclin-dependent kinase inhibitor 2A, p16, p16(INK4a)

---

Galerie de données de validation

• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (p16INK4a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C6, cellules Neuro-2a, cellules RAW 264.7, tissu utérin de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28416-1-AP cible Cdkn2a/p16INK4a dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cdkn2a/p16INK4a Protéine recombinante Ag28927
• Nom complet: cyclin-dependent kinase inhibitor 2A
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18-23 kDa
• Numéro d’acquisition GenBank: NM_001040654
• Symbole du gène: Cdkn2a
• Identification du gène (NCBI): 12578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers. The antibody is suitable for rats and mice.

Publications

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	Mol Neurobiol; Hypoxic Natural Killer Cells-Derived HIF-1α-Containing Exosomes Inhibit Cellular Senescence and Apoptosis in Neurocytes to Ameliorate Alzheimer's Disease by Eliminating Oxidative Damages; Authors - Yang Zhou

{{ptg:RelatedPrimaryAntibodies}}