Anticorps Polyclonal de lapin anti-p21

p21 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28248-1-PBS

Synonymes

Cdkn1a, Cdkn1, P21 protein, p21Cip1, p21WAF

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Galerie de données de validation

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  WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  WB analysis using 28248-1-AP (same clone as 28248-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28248-1-AP (p21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  IP result of anti-p21 (IP:28248-1-AP, 4ug; Detection:28248-1-AP 1:1000) with NIH/3T3 cells lysate 1920 ug. This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 28248-1-AP (p21 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 28248-1-AP (same clone as 28248-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using p21 antibody (28248-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 28248-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28248-1-PBS cible p21 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: p21 Protéine recombinante Ag28394
• Nom complet: cyclin-dependent kinase inhibitor 1A (P21)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: NM_001111099
• Symbole du gène: p21
• Identification du gène (NCBI): 12575
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

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