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Anticorps Monoclonal anti-p21

p21 Monoclonal Antibody for IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

souris

Applications

IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3C7D3

N° de cat : 67362-1-PBS

Synonymes

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

Galerie de données de validation

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using p21 Monoclonal antibody (67362-1-Ig)

    IF Staining of NIH/3T3 using 67362-1-Ig (same clone as 67362-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 67362-1-Ig (p21 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67362-1-PBS in a different storage buffer formulation.

Informations sur le produit

67362-1-PBS cible p21 dans les applications de IF/ICC, ELISA et montre une réactivité avec des échantillons souris

Réactivité souris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène p21 Protéine recombinante Ag28394
Nom complet cyclin-dependent kinase inhibitor 1A (P21)
Masse moléculaire calculée 18 kDa
Numéro d’acquisition GenBankNM_001111099
Symbole du gène p21
Identification du gène (NCBI) 12575
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

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