Anticorps Recombinant de lapin anti-p300

p300 Recombinant Antibody for IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(1)

CloneNo.

230369D12

N° de cat : 83078-5-RR

Synonymes

EP300, 230369D12, E1A-associated protein p300, EC:2.3.1.-, EC:2.3.1.48

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Galerie de données de validation

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  IP experiment of HeLa using 83078-5-RR

  IP result of anti-p300 (IP:83078-5-RR, 4ug; Detection:83078-5-RR 1:1000) with HeLa cells lysate 1050 ug.

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  IF Staining of A431 using 83078-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 83078-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p300 antibody (83078-5-RR, Clone: 230369D12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of THP-1 using 83078-5-RR

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug Anti-Human EP300 (83078-5-RR, Clone:230369D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  ChIP experiment of K-562 using 83078-5-RR

  Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of p300 (83078-5-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules A431, cellules HepG2
• Résultats positifs en FC (Intra): cellules THP-1,
• Résultats positifs en ChIP-qPCR: cellules K-562,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

83078-5-RR cible p300 dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: E1A binding protein p300
• Masse moléculaire calculée: 264 kDa
• Poids moléculaire observé: 300 kDa
• Numéro d’acquisition GenBank: NM_001429
• Symbole du gène: EP300
• Identification du gène (NCBI): 2033
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

p300 is a 300 kDa global transcriptional coactivator with histone acetyltransferase (HAT) activity. p300 binds to chromosomal histones and modulates the chromatin conformation by acetylation of its histones, leading to activation of various gene transcriptions. p300 plays a critical role in DNA repair synthesis after DNA damage induced by ultraviolet or radiation. p300 is overexpressed in various types of cancer, which is associated with worse patient outcomes (PMID: 27322077).

Publications

Species	Application	Title
human	WB	Cell Death Differ; GCLC desuccinylation regulated by oxidative stress protects human cancer cells from ferroptosis; Authors - Zixiang Chen

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