Anticorps Monoclonal anti-p38 MAPK

• Phare
• Validé par KD/KO

p38 MAPK Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

53

CloneNo.

1A1C2

Avis (1)

N° de cat : 66234-1-Ig

Synonymes

CSAID binding protein, CSBP, CSBP1, CSBP2, CSPB1, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAPK14, MAX interacting protein 2, Mxi2, p38, p38 MAPK, p38ALPHA, PRKM14, PRKM15, RK, SAPK2A

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Galerie de données de validation

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  WB analysis using 66234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66234-1-Ig

  WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells.

• 

  WB analysis of human heart using 66234-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66234-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66234-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66234-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules RAW 264.7, tissu cardiaque de porc, tissu cardiaque humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 52 publications below
• IHC: See 1 publications below

Informations sur le produit

66234-1-Ig cible p38 MAPK dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p38 MAPK Protéine recombinante Ag5797
• Nom complet: mitogen-activated protein kinase 14
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC031574
• Symbole du gène: MAPK14
• Identification du gène (NCBI): 1432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	J Hazard Mater; ERK/p38/ROS burst responses to environmentally relevant concentrations of diphenyl phosphate-evoked neutrophil extracellular traps formation: Assessing the role of autophagy.; Authors - Siwen Li
mouse	WB	Free Radic Biol Med; Cathelicidin-WA attenuates LPS-induced inflammation and redox imbalance through activation of AMPK signaling.; Authors - Weiche Wu
human	WB	Pharmacol Res; Anti-thrombotic effects mediated by dihydromyricetin involve both platelet inhibition and endothelial protection.; Authors - Shuai Chen
human	WB	Pharmacol Res; CX-5461 is a potent immunosuppressant which inhibits T cell-mediated alloimmunity via p53-DUSP5.; Authors - Guopin Pan
	WB	Development; FOXO1 mediates hypoxia-induced G0/G1 arrest in ovarian somatic granulosa cells by activating the TP53INP1-p53-CDKN1A pathway.; Authors - Chengyu Li
mouse	WB	Front Pharmacol; Comprehensive serum metabolomics and network analysis to reveal the mechanism of gypenosides in treating lung cancer and enhancing the pharmacological effects of cisplatin; Authors - Yan-Shuang Qi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Karen (Verified Customer) (11-10-2022)

p38MAPK detection for WB is not good, no bands detectable with different dilutions (1:500 and 1:1000). The same membrane was incubated with another p38MAPK antibody and was nicely visible.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 and 1:500
• Cell Tissue Type: HaCaTs

p38 MAPK Antibody Western Blot validation (1:1000 and 1:500 dilution) in HaCaTs (Cat no:66234-1-Ig)