Anticorps Monoclonal anti-p38 MAPK

• Phare
• Validé par KD/KO

p38 MAPK Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A1C2

N° de cat : 66234-1-PBS

Synonymes

MAPK14, CSPB1, CSBP2, CSBP1, CSBP

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Galerie de données de validation

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  WB analysis of HEK-293 using 66234-1-Ig (same clone as 66234-1-PBS)

  WB result of p38 MAPK antibody (66234-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p38 MAPK transfected HEK-293 cells. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66234-1-Ig (same clone as 66234-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66234-1-Ig (same clone as 66234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 66234-1-Ig (same clone as 66234-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66234-1-Ig (p38 MAPK Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66234-1-Ig (p38 MAPK antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66234-1-Ig (same clone as 66234-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using p38 MAPK antibody (66234-1-Ig, Clone: 1A1C2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66234-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50419-1

  Cytometric bead array standard curve of MP50419-1, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68965-1-PBS. Standard:Ag5797. Range: 0.195-200 ng/mL.

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  Cytometric bead array standard curve of MP50419-2

  Cytometric bead array standard curve of MP50419-2, p38 MAPK Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66234-1-PBS. Detection antibody: 68966-1-PBS. Standard:Ag5797. Range: 1.563-200 ng/mL.

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Informations sur le produit

66234-1-PBS cible p38 MAPK dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p38 MAPK Protéine recombinante Ag5797
• Nom complet: mitogen-activated protein kinase 14
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC031574
• Symbole du gène: p38 MAPK
• Identification du gène (NCBI): 1432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAPK14(mitogen-activated protein kinase 14) is also named as SAPK2A, p38MAPK, CSBP1, RK, p38, EXIP, Mxi2, CSBP2, PRKM14, PRKM15, CSPB1, p38ALPHA and belongs to the MAP kinase subfamily. MAPK14-signaling is a central pathway for the integration of instructive signals in dendritic cells for T(H)17 differentiation and inflammation(PMID:22231518). It plays an important role in the regulation of hematopoietic stem cell self-renewal in vitro and inhibition of MAPK14 activation with a small molecule inhibitor may represent a novel approach to promote ex vivo expansion of hematopoietic stem cell(PMID:21198398). This protein has 4 isoforms produced by alternative splicing.

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