Anticorps Polyclonal de lapin anti-AMACR/p504S

AMACR/p504S Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15918-1-AP

Synonymes

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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Galerie de données de validation

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  WB analysis using 15918-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15918-1-AP (AMACR/p504S antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15918-1-AP (AMACR/p504S antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 15918-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15918-1-AP (AMACR/p504S antibody at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules LNCaP, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

15918-1-AP cible AMACR/p504S dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMACR/p504S Protéine recombinante Ag8720
• Nom complet: alpha-methylacyl-CoA racemase
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC009471
• Symbole du gène: AMACR
• Identification du gène (NCBI): 23600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and it can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IHC	Front Cell Dev Biol; SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer.; Authors - Zhao-Ming Xiao
human	IHC	Oncol Lett; The auxiliary diagnostic value of prostate-specific antigen and α-methylacyl-CoA racemase in prostate cancer.; Authors - Daijun Yang
human	IHC	Andrologia; Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.; Authors - Xiqi Ding
human	WB,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham

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