Anticorps Monoclonal anti-p504S,AMACR

p504S,AMACR Monoclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

2H9B5

N° de cat : 60240-1-Ig

Synonymes

2 methylacyl CoA racemase, alpha methylacyl CoA racemase, AMACR, CBAS4, p504S, p504S,AMACR, RACE

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Galerie de données de validation

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  WB analysis using 60240-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 60240-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60240-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HepG2 using 60240-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human p504S,AMACR (60240-1-Ig, Clone:2H9B5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules 4T1, cellules HEK-293, cellules HeLa, cellules HSC-T6, cellules LNCaP, cellules NIH/3T3, cellules PC-3
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

60240-1-Ig cible p504S,AMACR dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p504S,AMACR Protéine recombinante Ag8720
• Nom complet: alpha-methylacyl-CoA racemase
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC009471
• Symbole du gène: AMACR
• Identification du gène (NCBI): 23600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).