Anticorps Monoclonal anti-AMACR/p504S

AMACR/p504S Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H9B5

N° de cat : 60240-1-PBS

Synonymes

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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Galerie de données de validation

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  WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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  WB analysis using 60240-1-Ig (same clone as 60240-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60240-1-Ig (same clone as 60240-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60240-1-Ig (p504S,AMACR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60240-1-Ig (same clone as 60240-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using p504S,AMACR antibody (60240-1-Ig, Clone: 2H9B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60240-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60240-1-PBS cible AMACR/p504S dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMACR/p504S Protéine recombinante Ag8720
• Nom complet: alpha-methylacyl-CoA racemase
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC009471
• Symbole du gène: AMACR
• Identification du gène (NCBI): 23600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

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