Anticorps Recombinant de lapin anti-AMACR/p504S

AMACR/p504S Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242456D1

N° de cat : 84891-2-PBS

Synonymes

AMACR, p504S, 242456D1, 2-methylacyl-CoA racemase, Alpha-methylacyl-CoA racemase

---

Galerie de données de validation

• 

  WB analysis using 84891-2-RR (same clone as 84891-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84891-2-RR (AMACR/p504S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84891-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 84891-2-RR (same clone as 84891-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84891-2-RR (AMACR/p504S antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84891-2-PBS in a different storage buffer formulation.

---

Informations sur le produit

84891-2-PBS cible AMACR/p504S dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: AMACR/p504S Protéine recombinante Ag8720
• Nom complet: alpha-methylacyl-CoA racemase
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC009471
• Symbole du gène: AMACR
• Identification du gène (NCBI): 23600
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

AMACR(Alpha-methyl acyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. A mitochondrial and peroxisomal enzyme catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing and can form a dimer of 70-75 kDa(PMID:21812041). Defects in AMACR cause alpha-methyl acyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

{{ptg:RelatedPrimaryAntibodies}}