Anticorps Monoclonal anti-AMACR/p504S

AMACR/p504S Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H9B5

N° de cat : CL488-60240

Synonymes

AMACR, p504S, EC:5.1.99.4, Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase

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Galerie de données de validation

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  IF Staining of A431 using CL488-60240

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 p504S,AMACR antibody (CL488-60240, Clone: 2H9B5 ) at dilution of 1:200.

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  FC experiment of LNCaP using CL488-60240

  1X10^6 LNCaP cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human p504S,AMACR (CL488-60240, Clone:2H9B5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules LNCaP,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60240 cible AMACR/p504S dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AMACR/p504S Protéine recombinante Ag8720
• Nom complet: alpha-methylacyl-CoA racemase
• Masse moléculaire calculée: 382 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC009471
• Symbole du gène: AMACR
• Identification du gène (NCBI): 23600
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMACR(Alpha-methylacyl-CoA racemase) belongs to the CaiB/BaiF CoA-transferase family. It is a mitochondrial and peroxisomal enzyme that catalyzes the conversion of 2R stereoisomers of phytanic and pristanic acid to their S counterparts. AMACR has previously been shown to be a highly sensitive marker for colorectal and clinically localized prostate cancer (PCa). However, AMACR expression is down-regulated at the transcript and protein level in hormone-refractory metastatic PCa, suggesting a hormone-dependent expression of AMACR(PMID:12213712). It has 3 isoforms produced by alternative splicing. Defects in AMACR are the cause of alpha-methylacyl-CoA racemase deficiency (AMACRD) and congenital bile acid synthesis defect type 4 (CBAS4).

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