Anticorps Monoclonal anti-p57Kip2

p57Kip2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

1A12H3

N° de cat : 66794-1-Ig

Synonymes

CDKN1C, KIP2, p57, Cyclin-dependent kinase inhibitor p57, Cyclin-dependent kinase inhibitor 1C

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Galerie de données de validation

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  WB analysis using 66794-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66794-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66794-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66794-1-Ig (CDKN1C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66794-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66794-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66794-1-Ig (p57Kip2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules HCT 116, cellules HeLa, cellules MDA-MB-231, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below

Informations sur le produit

66794-1-Ig cible p57Kip2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p57Kip2 Protéine recombinante Ag19927
• Nom complet: cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• Masse moléculaire calculée: 316 aa, 32 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC067842
• Symbole du gène: p57 Kip2/CDKN1C
• Identification du gène (NCBI): 1028
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CDKN1C, also named as p57KIP2 and KIP2, belongs to the CDI family. CDKN1C is a potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. It is a negative regulator of cell proliferation. CDKN1C may play a role in maintenance of the non-proliferative state throughout life.

Publications

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	Nat Commun; The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.; Authors - Giovanna Rigillo
mouse	WB	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou
human	WB	J Transl Med; STK40 inhibits trophoblast fusion by mediating COP1 ubiquitination to degrade P57Kip2; Authors - Xia Li
	WB	bioRxiv; D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability; Authors - Gergely Rona
human	WB	Mol Cell; CDK-independent role of D-type cyclins in regulating DNA mismatch repair; Authors - Gergely Rona

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