Anticorps Monoclonal anti-p70(S6K)

p70(S6K) Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D11C6

N° de cat : 66638-1-PBS

Synonymes

Phospho-p70(S6K) (Thr389), p70 S6K, p70 S6 kinase, RPS6KB1, p70 S6K 1

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Galerie de données de validation

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  WB analysis of Jurkat using 66638-1-Ig (same clone as 66638-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66638-1-Ig (same clone as 66638-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66638-1-Ig (p70(S6K) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66638-1-Ig (same clone as 66638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66638-1-Ig (same clone as 66638-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66638-1-Ig (p70(S6K) antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66638-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50673-1

  Cytometric bead array standard curve of MP50673-1, p70(S6K) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66638-1-PBS. Detection antibody: 66638-2-PBS. Standard:Ag5883. Range: 1.563-100 ng/mL.

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Informations sur le produit

66638-1-PBS cible p70(S6K) dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: p70(S6K) Protéine recombinante Ag5883
• Nom complet: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 60-75 kDa
• Numéro d’acquisition GenBank: BC053365
• Symbole du gène: p70 S6K
• Identification du gène (NCBI): 6198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of MTOR and acts as a crucial effector of MTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). RPS6KB1 is a 70 kDa protein and has 5 isoforms with the calculated molecular mass of 51-59kDa produced by alternative initiation.

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