Anticorps Recombinant de lapin anti-pan-RAS

pan-RAS Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2I22

N° de cat : 81615-1-PBS

Synonymes

KRAS, pan RAS, EC:3.6.5.2, c-K-ras, c-Ki-ras

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Galerie de données de validation

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  WB analysis using 81615-1-RR (same clone as 81615-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81615-1-RR (pan-RAS antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81615-1-RR (same clone as 81615-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81615-1-RR (pan-RAS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81615-1-RR (same clone as 81615-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using pan-RAS antibody (81615-1-RR, Clone: 2I22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81615-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP80009-1

  Cytometric bead array standard curve of MP80009-1, KRAS Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 60309-1-PBS. Detection antibody: 81615-1-PBS. Standard: Ag2700. Range: 0.391-50 ng/mL.

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  Affinity and Kinetic Characterization of 81615-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 81615-1-PBS against Human pan-RAS was performed. The affinity constant is 2.45 nM.

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  Affinity and Kinetic Characterization of 81615-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81615-1-RR against Human pan-RAS were performed. The affinity constant is below 1 pM.

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Informations sur le produit

81615-1-PBS cible pan-RAS dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: pan-RAS Protéine recombinante Ag2700
• Nom complet: v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• Masse moléculaire calculée: 188 aa, 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC013572
• Symbole du gène: KRAS
• Identification du gène (NCBI): 3845
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) belong to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. K-Ras, H-Ras, and N-Ras have similar structure and sequences. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. The ras genes are ubiquitously expressed although mRNA analysis suggests different level expression in tissue. Mutations in each ras gene frequently were found in different tumors, suggesting their involvement in the development of specific neoplasia. This antibody can recognize K-Ras, H-Ras, and N-Ras.

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