Anticorps Polyclonal de lapin anti-pan-keratin

pan-keratin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 26411-1-AP

Synonymes

pan Cytokeratin, pan-ck, pan-cytokeratin, pan-keratin

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Galerie de données de validation

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  WB analysis using 26411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26411-1-AP (pan-keratin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens)..

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  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens)..

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  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

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  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

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  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 26411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 26411-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human pan-keratin (26411-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu œsophagien humain, tissu d'amygdalite humain, tissu de cancer du poumon humain, tissu de carcinome à cellules rénales humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du côlon humain, cellules A431, cellules HeLa
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:50000-1:200000
• Immunohistochimie (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 12 publications below
• IF: See 10 publications below

Informations sur le produit

26411-1-AP cible pan-keratin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: pan-keratin Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Poids moléculaire observé: 46-58 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: KRT5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

Publications

Species	Application	Title
human	IF	Biomaterials; Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements.; Authors - Hiroyoshi Y Tanaka
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
human	IF	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
mouse	IHC	Oncogene; USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.; Authors - Juntae Kwon
human	IF	Front Cell Dev Biol; Preclinical Application of Conditional Reprogramming Culture System for Laryngeal and Hypopharyngeal Carcinoma; Authors - Yanbo Dong
human	IHC	J Cancer Res Clin Oncol; B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy; Authors - Siji Chen