Anticorps Polyclonal de lapin anti-Pan-Keratin

Pan-Keratin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(46)

N° de cat : 26411-1-AP

Synonymes

Pan CK, Pan Keratin, pan-CK, CK 5, CK-5

---

Galerie de données de validation

• 

  WB analysis using 26411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26411-1-AP (pan-keratin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens)..

• 

  IHC staining of human oesophagus using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens)..

• 

  IHC staining of human cervical cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (Pan-Keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (Pan-Keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brown disease using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (Pan-Keratin antibody) at dilution of 1:3000 (under 20x lens).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (Pan-Keratin antibody) at dilution of 1:3000 (under 20x lens).

• 

  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26411-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26411-1-AP (pan-keratin antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

• 

  IF Staining of human colon cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD8 antibody (66868-1-Ig, Clone: 1G2B10, green).

• 

  IF Staining of human rectal cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human rectal cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human rectal cancer tissue using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 26411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse breast cancer using 26411-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse breast cancer using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A431 using 26411-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using pan-keratin antibody (26411-1-AP) at dilution of 1:250 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• 

  IF Staining of HeLa using 26411-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using pan-keratin antibody (26411-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu œsophagien humain, tissu d'amygdalite humain, tissu de cancer du col de l'utérus humain, tissu de cancer du poumon humain, tissu de carcinome à cellules rénales humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du côlon humain, human rectal cancer tissue
• Résultats positifs en IF/ICC: cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:50000-1:200000
• Immunohistochimie (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 15 publications below
• IF: See 25 publications below

Informations sur le produit

26411-1-AP cible Pan-Keratin dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pan-Keratin Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Poids moléculaire observé: 46-58 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: Cytokeratin 5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pan-keratin, also known as pan-cytokeratin, refers to a group of antibodies that recognize a broad array of keratin proteins. Keratins are a large family of intermediate filament (IF) proteins that provide structural integrity to epithelial cells. These proteins are essential for maintaining cell shape, strength, and resilience . The keratin gene family is divided into two major groups: type I keratins (acidic) and type II keratins (basic), with 27 type I and 26 type II keratin genes in humans. Pan-keratin immunostaining is widely used in pathology and research due to its ability to detect a variety of keratin proteins, making it a valuable tool for identifying epithelial cells and tissues. This staining technique is particularly useful in the diagnosis of tumors, as it can help distinguish between epithelial and non-epithelial neoplasms. This antibody is a pan-keratin antibody.

Publications

Species	Application	Title
human	IF	Nat Commun; The estrogen response in fibroblasts promotes ovarian metastases of gastric cancer; Authors - Simeng Hu
human	IF	Biomaterials; Heterotypic 3D pancreatic cancer model with tunable proportion of fibrotic elements.; Authors - Hiroyoshi Y Tanaka
mouse	IF	Biomaterials; MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.; Authors - Tian Wang
	IHC	J Nanobiotechnology; ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis; Authors - Xuan Lai
human	IF	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li
mouse	IHC	Oncogene; USP13 promotes development and metastasis of high-grade serous ovarian carcinoma in a novel mouse model.; Authors - Juntae Kwon

{{ptg:RelatedPrimaryAntibodies}}