Anticorps Recombinant de lapin anti-pan-keratin

pan-keratin Recombinant Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

7J23

N° de cat : 82548-3-RR

Synonymes

CK-PAN, pan Cytokeratin, pan keratin, pan-Cytokeratin, pan-keratin

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Galerie de données de validation

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82548-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 82548-3-RR (KRT5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 82548-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using KRT5 antibody (82548-3-RR, Clone: 7J23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82548-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using KRT5 antibody (82548-3-RR, Clone: 7J23 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82548-3-RR cible pan-keratin dans les applications de IF, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: pan-keratin Protéine recombinante Ag24184
• Nom complet: keratin 5
• Masse moléculaire calculée: 590 aa, 62 kDa
• Numéro d’acquisition GenBank: BC024292
• Symbole du gène: Cytokeratin 5
• Identification du gène (NCBI): 3852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. This antibody is a pan-keratin antibody.

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