Anticorps Polyclonal de lapin anti-Peroxiredoxin 2

• Phare
• Validé par KD/KO

Peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10545-2-PBS

Synonymes

PRDX2, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB, NKEF-B

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Galerie de données de validation

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  WB analysis using 10545-2-AP (same clone as 10545-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (Peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  WB result of Peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  IP result of anti-Peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug. This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (Peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (Peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (Peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 10545-2-AP (same clone as 10545-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (Peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10545-2-AP (same clone as 10545-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10545-2-AP (same clone as 10545-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10545-2-PBS in a different storage buffer formulation.

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Informations sur le produit

10545-2-PBS cible Peroxiredoxin 2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peroxiredoxin 2 Protéine recombinante Ag0835
• Nom complet: peroxiredoxin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003022
• Symbole du gène: peroxiredoxin 2
• Identification du gène (NCBI): 7001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

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