Anticorps Monoclonal anti-Peroxiredoxin 2

• Phare
• Validé par KD/KO

Peroxiredoxin 2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3H6C4

N° de cat : 60202-1-PBS

Synonymes

PRDX2, 3H6C4, EC:1.11.1.24, Natural killer cell-enhancing factor B, NKEFB

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Galerie de données de validation

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  WB analysis using 60202-1-Ig (same clone as 60202-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60202-1-Ig (same clone as 60202-1-PBS)

  WB result of Peroxiredoxin 2 antibody (60202-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 60202-1-Ig (same clone as 60202-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 60202-1-Ig(peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60202-1-Ig (Peroxiredoxin 2 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 60202-1-Ig(Peroxiredoxin 2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 60202-1-Ig (same clone as 60202-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Peroxiredoxin 2 antibody (60202-1-Ig, Clone: 3H6C4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60202-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60202-1-PBS cible Peroxiredoxin 2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peroxiredoxin 2 Protéine recombinante Ag0835
• Nom complet: peroxiredoxin 2
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC003022
• Symbole du gène: peroxiredoxin 2
• Identification du gène (NCBI): 7001
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

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