Anticorps Polyclonal de lapin anti-Phospho-TDP43 (Ser409/410)
Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (2)
Applications
WB, IP, IHC, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 22309-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa traitées à la calyculine A, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 33 publications below |
| IHC | See 38 publications below |
| IF | See 27 publications below |
| IP | See 1 publications below |
Informations sur le produit
22309-1-AP cible Phospho-TDP43 (Ser409/410) dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | rat, Humain, singe, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | TAR DNA binding protein |
| Masse moléculaire calculée | 43 kDa |
| Poids moléculaire observé | 40-50 kDa, 25-35 kDa |
| Numéro d’acquisition GenBank | NM_007375 |
| Symbole du gène | TARDBP |
| Identification du gène (NCBI) | 23435 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-TDP43 (Ser409/410) antibody 22309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Med The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. | ||
Nat Neurosci TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration. | ||
JAMA Neurol TDP-43 Accumulation Within Intramuscular Nerve Bundles of Patients With Amyotrophic Lateral Sclerosis. | ||
Sci Adv Calpain-2 as a therapeutic target in repeated concussion-induced neuropathy and behavioral impairment. | ||
Acta Neuropathol Synapse loss in the prefrontal cortex is associated with cognitive decline in amyotrophic lateral sclerosis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shenyi (Verified Customer) (04-27-2022) | Staining with this antibody is very different from with the recombinant one (80007-1-RR) which I received as a free test vial. It showed strong signal in the nucleus while the recombinant one only showed signal in the cytoplasm. Now I'm confused: Could pTDP-43 (Ser409/410) also present in nucleus or it's the antibody being nonspecific?
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FH Azita (Verified Customer) (03-10-2020) | Immunofluorescent analysis of (4% PFA fixed) NSC-34 cells, using p- (109/110) TDP-43 antibody at 1/500 dilution and Alexa Flour conjugated Donkey anti Rabbit IgG (H+L) (Red). Bisbenzimide (Blue) labels cell nuclei.
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FH Laura (Verified Customer) (01-10-2020) | Works well at this concentration on HEK293T lysate.
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FH Owen (Verified Customer) (10-30-2019) | This antibody was used to image TDP43 in tissue and cell samples. The antibody was useful to image such a difficult target.
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