Anticorps Recombinant de lapin anti-Phospho-TDP43 (Ser409/410)

Phospho-TDP43 (Ser409/410) Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(21)

CloneNo.

6M10

N° de cat : 80007-1-RR

Synonymes

TARDBP, TDP 43, TDP43, TDP-43, TAR DNA-binding protein 43



Applications testées

Résultats positifs en WBcellules HeLa, Calyculin A treated cells
Résultats positifs en IHCcortex frontal d'un cas de DLFT-TDP de type B, tissu cérébral humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

80007-1-RR cible Phospho-TDP43 (Ser409/410) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéeHumain, poisson-zèbre, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet TAR DNA binding protein
Masse moléculaire calculée 43 kDa
Poids moléculaire observé 45-50 kDa
Numéro d’acquisition GenBankNM_007375
Symbole du gène TDP-43
Identification du gène (NCBI) 23435
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 80007-1-RR is a recombinant rabbit monoclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

Protocole

Product Specific Protocols
WB protocol for Phospho-TDP43 (Ser409/410) antibody 80007-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biol Chem

Regulation of TAR DNA binding protein 43 (TDP-43) homeostasis by cytosolic DNA accumulation

Authors - Cha Yang
WB

Biochim Biophys Acta Mol Basis Dis

Phosphorylated TAR DNA-Binding Protein-43: Aggregation and Antibody-Based Inhibition.

Authors - Josephine C Esposto
humanWB,IF

bioRxiv

TDP-43 pathology links innate and adaptive immunity in amyotrophic lateral sclerosis

Authors - Baggio A Evangelista
human,mouseIF

bioRxiv

Fructose-2,6-bisphosphate restores TDP-43 pathology-driven genome repair deficiency in motor neuron diseases

Authors - Anirban Chakraborty
mouseWB,IF

Ecotoxicol Environ Saf

Arsenic exposure activates microglia, inducing neuroinflammation and promoting the occurrence and development of Alzheimer's disease-like neurodegeneration in mice

Authors - Bo Zhang
mouseIF

Res Sq

Endogenous TDP-43 mislocalization in a novel knock-in mouse model reveals DNA repair impairment, inflammation, and neuronal senescence

Authors - Joy Mitra

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Rashmi (Verified Customer) (02-14-2025)

used for Western blot, IHC, highly recommended

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 , 1:2000
  • Cell Tissue Type: HEK293T
FH

Silvia (Verified Customer) (08-21-2020)

Excellent phospho-specific TDP-43 antibody. Tested by IHC in ethanol and NBF fixed FTLD-TDP brains and ALS spinal cord. The antibody works well with citrate buffer and FA as antigen retrieval. The dilutions that worked better showing high specificity and low background ranged from 1,000-5,000.Works great also by western blot at dilutions 1:1,000-1:2,000.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000)
  • Cell Tissue Type: human cortex and spinal cord
Phospho-TDP43 (Ser409/410) Antibody Western Blot,Immunohistochemistry,Immunofluorescence validation (Wblot (1:1,000-2,000) IHC (1:1,500 - 1:5,000) dilution) in human cortex and spinal cord (Cat no:80007-1-RR)
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