Anticorps Monoclonal anti-Phospho-Akt (Tyr315)

Phospho-Akt (Tyr315) Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF,ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

5C4D12

N° de cat : 60072-1-Ig

Synonymes

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Galerie de données de validation

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Applications testées

Dilution recommandée

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IF: See 1 publications below

Informations sur le produit

60072-1-Ig cible Phospho-Akt (Tyr315) dans les applications de WB, IF,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Phospho Peptide
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: NM_005163
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase B (PKB/Akt) has been well established as an important signaling intermediate, and its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337). Akt, also known as protein kinase B (PKB), is a serine/threonine kinase activated downstream of class I phosphatidylinositol 3-kinase (PI3K) and various receptors. It has emerged as the key regulator for many signal transduction pathways, modulating multiple processes such as cell survival, proliferation, growth, angiogenesis, and glucose metabolism(PMID:16139227). Its deregulation has been implicated in the development of human cancer and diabetes(PMID:12176337).

Publications

Species	Application	Title
human	WB	Sci Rep; TCF7L2 involvement in estradiol- and progesterone-modulated islet and hepatic glucose homeostasis.; Authors - Fengqin Dong
rat	WB	Neuropharmacology; Puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition.; Authors - Nan Wang
human	WB	Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin
human	WB	PLoS One; Ubiquitin B in cervical cancer: critical for the maintenance of cancer stem-like cell characters.; Authors - Yuan Tian
human	WB	Apoptosis; SZC017, a novel oleanolic acid derivative, induces apoptosis and autophagy in human breast cancer cells.; Authors - Lei Gao
human	WB	Biochim Biophys Acta; Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation.; Authors - Jiawei Guan