Anticorps Monoclonal anti-Alpha smooth muscle actin specific

Alpha smooth muscle actin specific Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA

Conjugaison

Non conjugué

Publications(200)

CloneNo.

1E9A11

N° de cat : 67735-1-Ig

Synonymes

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 1E9A11

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Galerie de données de validation

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  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67735-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig vein using 67735-1-Ig

  pig vein tissue were subjected to SDS PAGE followed by western blot with 67735-1-Ig (a-SMA specific antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67735-1-Ig (smooth muscle actin specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 67735-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67735-1-Ig (a-SMA specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat heart using 67735-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using a-SMA specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of mouse breast cancer using 67735-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Alpha smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  IF Staining of C2C12 using 67735-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using smooth muscle actin specific antibody (67735-1-Ig, Clone: 1E9A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of C2C12 using 67735-1-Ig

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human smooth muscle actin specific (67735-1-Ig, Clone:1E9A11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de rat, tissu de côlon de porc, tissu de côlon de rat, tissu de côlon de souris, tissu d'estomac de lapin, tissu d'estomac de porc, tissu veineux de porc
• Résultats positifs en IHC: tissu de côlon humain, tissu d'appendicite humain, tissu de cancer du foie humain, tissu de côlon de rat, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de rat,
• Résultats positifs en IF-Fro: mouse breast cancer,
• Résultats positifs en IF/ICC: cellules C2C12,
• Résultats positifs en FC (Intra): cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 90 publications below
• IHC: See 49 publications below
• IF: See 122 publications below
• CoIP: See 1 publications below

Informations sur le produit

67735-1-Ig cible Alpha smooth muscle actin specific dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: NM_001613
• Symbole du gène: Alpha smooth muscle actin
• Identification du gène (NCBI): 59
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
mouse	IF	Adv Mater; A "Bridge-Building" Glycan Scaffold Mimicking Microbial Invasion for In Situ Endothelialization.; Authors - Ruoyu Mu
mouse	WB	Protein Cell; Neutrophil extracellular traps license macrophage production of chemokines to facilitate CD8+ T cell infiltration in obstruction-induced renal fibrosis; Authors - Hongshuai Jia
mouse	IF	Nat Commun; ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice; Authors - Tao Zhuang
human	IHC	Mol Cancer; circFARP1 enables cancer-associated fibroblasts to promote gemcitabine resistance in pancreatic cancer via the LIF/STAT3 axis.; Authors - Chonghui Hu
human	IF	Adv Sci (Weinh); Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Esophageal Squamous Cell Carcinoma; Authors - Yongxu Jia
mouse	IF	Acta Pharm Sin B; ADAR1 regulates vascular remodeling in hypoxic pulmonary hypertension through N1-methyladenosine modification of circCDK17; Authors - Junting Zhang

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