Anticorps Polyclonal de lapin anti-SARS-CoV-2 S protein (944-1214 aa)

SARS-CoV-2 S protein (944-1214 aa) Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus et plus (4)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 28867-1-AP

Synonymes

S protein, SARS-CoV-2 Spike Glycoprotein (944-1214 aa), spike, spike protein

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Galerie de données de validation

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  ELISA experiment of Recombinant protein using 28867-1-AP

  SARS-CoV-2 Spike Antibody (28867-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28867-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

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Applications testées

• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Test immuno-enzymatique (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

28867-1-AP cible SARS-CoV-2 S protein (944-1214 aa) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Réactivité citée: Humain, singe, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 S protein (944-1214 aa) Protéine recombinante Ag30685
• Nom complet: SARS-CoV-2 Spike Protein
• Masse moléculaire calculée: 141 kDa
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 S Protein
• Identification du gène (NCBI): 43740568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). This antibody detects the spike protein of SARS and SARS-COV-2.

Publications

Species	Application	Title
monkey,hamster	IF	Nat Biomed Eng; High-throughput screening of genetic and cellular drivers of syncytium formation induced by the spike protein of SARS-CoV-2; Authors - Charles W F Chan
human	IF	Nat Immunol; Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules.; Authors - Matteo Stravalaci
	IF	Cell Rep; Diverse immunoglobulin gene usage and convergent epitope targeting in neutralizing antibody responses to SARS-CoV-2.; Authors - Xiaojuan Zhou
	IF	Pathogens; Hydrophobic Alpha-Helical Short Peptides in Overlapping Reading Frames of the Coronavirus Genome; Authors - Takashi Okura
mouse	IHC,WB	Nat Commun; Essential role of TMPRSS2 in SARS-CoV-2 infection in murine airways; Authors - Naoko Iwata-Yoshikawa
human	IF	Int J Mol Sci; Modulation of NBAS-Related Functions in the Early Response to SARS-CoV-2 Infection; Authors - Valentina Granata