Anticorps Polyclonal de lapin anti-SARS-CoV-2 S protein (428-506 aa)

SARS-CoV-2 S protein (428-506 aa) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 28901-1-AP

Synonymes

SARS-CoV-2 Spike Glycoprotein (428-506 aa)

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Galerie de données de validation

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  WB analysis using 28901-1-AP

  Spike Glycoprotein with the domain 1-658aa expressed in HEK-293 cells were subjected to SDS PAGE followed by western blot with 28901-1-AP (SARS-CoV-2 Spike Glycoprotein (428-506 aa) Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  ELISA experiment of Recombinant protein using 28901-1-AP

  SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Glycoprotein (428-506aa) Antibody (28901-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. .

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Applications testées

• Résultats positifs en WB: Protéine recombinante
• Résultats positifs en ELISA: Protéine recombinante

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Test immuno-enzymatique (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28901-1-AP cible SARS-CoV-2 S protein (428-506 aa) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SARS-CoV-2 S protein (428-506 aa) Protéine recombinante Ag30684
• Nom complet: SARS-CoV-2 Spike Protein
• Masse moléculaire calculée: 141 kDa
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: COVID-19 S Protein
• Identification du gène (NCBI): 43740568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Irene (Verified Customer) (10-15-2020)

Tested the antibody for IF and western blot and it did not work for either application. There were minimal colocalization but also non-specific binding for IF.

• Applications: Western Blot, Immunofluorescence