Anticorps Recombinant de lapin anti-SARS-CoV-2 Spike

SARS-CoV-2 Spike Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

4I18

N° de cat : 82734-4-RR

Synonymes

COVID-19 RBD, RBD, Spike, spike protein, S protein

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Galerie de données de validation

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  WB analysis of Recombinant protein using 82734-4-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 82734-4-RR (spike protein antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82734-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82734-4-RR against Virus spike protein were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82734-4-RR cible SARS-CoV-2 Spike dans les applications de WB, ELISA et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SARS-CoV-2 Spike Protéine recombinante Ag30684
• Nom complet: SARS-CoV-2 Spike Protein
• Masse moléculaire calculée: 141 kDa
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: SARS-COV-2
• Identification du gène (NCBI): 43740568
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.

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