Anticorps Recombinant de lapin anti-spike protein

spike protein Recombinant Antibody for WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

WB

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

4I18

N° de cat : CL750-82734-4

Synonymes

COVID-19 RBD, RBD, Spike, S protein, SARS CoV 2

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Galerie de données de validation

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  WB analysis using CL750-82734-4

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82734-4 (spike protein antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: Protéine recombinante,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-82734-4 cible spike protein dans les applications de WB et montre une réactivité avec des échantillons Virus

• Réactivité: Virus
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: spike protein Protéine recombinante Ag30684
• Nom complet: SARS-CoV-2 Spike Protein
• Masse moléculaire calculée: 141 kDa
• Numéro d’acquisition GenBank: NC_045512
• Symbole du gène: SARS-COV-2
• Identification du gène (NCBI): 43740568
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa.

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