Anticorps Recombinant de lapin anti-transgelin/SM22

transgelin/SM22 Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

240864A11

N° de cat : 83922-2-RR

Synonymes

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

---

Galerie de données de validation

• 

  WB analysis using 83922-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 83922-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 83922-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 83922-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 83922-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 83922-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

---

Applications testées

• Résultats positifs en WB: tissu de côlon de souris, tissu de côlon de rat, tissu d'estomac de rat, tissu d'estomac de souris
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83922-2-RR cible transgelin/SM22 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: transgelin/SM22 Protéine recombinante Ag0764
• Nom complet: transgelin
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 19-22 kDa
• Numéro d’acquisition GenBank: BC004927
• Symbole du gène: transgelin/SM22
• Identification du gène (NCBI): 6876
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.

{{ptg:RelatedPrimaryAntibodies}}