Anticorps Recombinant de lapin anti-uPAR/CD87

uPAR/CD87 Recombinant Antibody for IF/ICC, FC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC

Conjugaison

Non conjugué

CloneNo.

240366G10

N° de cat : 98048-1-RR

Synonymes

PLAUR, UPAR, U PAR, MO3, CD87

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Galerie de données de validation

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  IF Staining of HeLa using 98048-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using uPAR/CD87 antibody (98048-1-RR, Clone: 240366G10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of U-937 using 98048-1-RR

  1x10^6 PMA-treated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) (red) or isotype control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated U-937 cells were surface stained with 0.25 ug Anti-Human uPAR/CD87 Rabbit Recombinant Antibody (98048-1-RR, Clone: 240366G10) and PE-conjugated Goat Anti-Rabbit IgG (black). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98048-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98048-1-RR against Human uPAR were performed. The affinity constant is 4.70 nM.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en cytométrie: PMA treated U-937 cells,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

98048-1-RR cible uPAR/CD87 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: NM_002659.4
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

uPAR, also known as CD87, is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

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