Anticorps Monoclonal anti-uPAR/CD87

uPAR/CD87 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2A3E8

N° de cat : 68727-1-Ig

Synonymes

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

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Galerie de données de validation

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  WB analysis of U2OS using 68727-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68727-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68727-1-Ig (uPAR, PLAUR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung squamous cell cancer using 68727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung squamous cell cancer using 68727-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung squamous cell cancer slide using 68727-1-Ig (uPAR/CD87 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A549, cellules U-251
• Résultats positifs en IHC: human lung squamous cell cancer, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68727-1-Ig cible uPAR/CD87 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: uPAR/CD87 Protéine recombinante Ag28056
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-50 kDa
• Numéro d’acquisition GenBank: BC002788
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

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