Anticorps Recombinant de lapin anti-uPAR/CD87

uPAR/CD87 Recombinant Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

230148A1

N° de cat : 82953-1-RR

Synonymes

uPAR, PLAUR, PLAUR, 230148A1, CD87, MO3

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Galerie de données de validation

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  IHC staining of mouse brain using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 82953-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82953-1-RR (uPAR/CD87 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-1080 using 82953-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using uPAR/CD87 antibody (82953-1-RR, Clone: 230148A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 82953-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-1-RR against Human uPAR, PLAUR were performed. The affinity constant is 1.49 nM.

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Applications testées

• Résultats positifs en IHC: human ovary cancer tissue, tissu cérébral de souris, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HT-1080,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82953-1-RR cible uPAR/CD87 dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: uPAR/CD87 Protéine recombinante Ag28056
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Numéro d’acquisition GenBank: BC002788
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

uPAR is a 45-65 kDa, highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

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