Anticorps Recombinant de lapin anti-uPAR/CD87

uPAR/CD87 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

230148B10

N° de cat : 82953-2-RR

Synonymes

uPAR, PLAUR, PLAUR, UPAR, U PAR, MO3

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Galerie de données de validation

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  WB analysis using 82953-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 82953-2-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82953-2-RR (uPAR/CD87 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 82953-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82953-2-RR against Human uPAR, PLAUR were performed. The affinity constant is 0.527 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules DU 145, cellules PC-3, cellules THP-1
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, tissu de cancer du poumon humain, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82953-2-RR cible uPAR/CD87 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: uPAR/CD87 Protéine recombinante Ag28056
• Nom complet: PLAUR
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-50 kDa
• Numéro d’acquisition GenBank: BC002788
• Symbole du gène: uPAR
• Identification du gène (NCBI): 5329
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

uPAR is a highly glycosylated, GPI-anchored membrane protein. In addition to the membrane-anchored form, uPAR is released from the plasma membrane by cleavage of the GPI anchor and can be found as a soluble form (suPAR). uPAR contains three homologous domains (D1-D3) of which the N-terminal one (D1) represents the uPA-binding domain. After binding to uPAR, uPA cleaves plasminogen, generating the active protease plasmin which is involved in a wide variety of physiologic and pathologic processes. In addition to regulating proteolysis, uPAR has important function in cell adhesion, migration and proliferation. Studies reveal that uPAR expression is elevated during inflammation and tissue remodeling and in many human cancers, in which it frequently indicates poor prognosis. (PMID 20027185; 12461559)

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