Anticorps Monoclonal anti-ubiquitin

ubiquitin Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1D7B2

N° de cat : 60310-1-Ig

Synonymes

ubiquitin

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Galerie de données de validation

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  WB analysis of HeLa using 60310-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60310-1-Ig (ubiquitin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60310-1-Ig (ubiquitin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 60310-1-Ig

  Immunofluorescent analysis of HeLa cells using 60310-1-Ig (ubiquitin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60310-1-Ig cible ubiquitin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ubiquitin Protéine recombinante Ag0260
• Nom complet: ubiquitin B
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000379
• Symbole du gène: UBB
• Identification du gène (NCBI): 7314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publications

Species	Application	Title
human		Cancer Lett; Exosome-mediated transfer of SNHG7 enhances docetaxel resistance in lung adenocarcinoma; Authors - Kai Zhang
	WB	Cell Death Differ; Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-β hydroxysteroid dehydrogenase type 10.; Authors - G Bertolin
human	WB	Autophagy; Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples; Authors - Jens O. Watzlawik