Anticorps Monoclonal anti-ubiquitin

ubiquitin Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1D7B2

N° de cat : 60310-1-Ig

Synonymes

ubiquitin B, UBB, Polyubiquitin-B, Polyubiquitin B

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Galerie de données de validation

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Applications publiées

• WB: See 3 publications below

Informations sur le produit

60310-1-Ig cible ubiquitin dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ubiquitin Protéine recombinante Ag0260
• Nom complet: ubiquitin B
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000379
• Symbole du gène: ubiquitin
• Identification du gène (NCBI): 7314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ubiquitin B (UBB) is a member of ubiquitin family, one of the most conserved proteins known. Ubiquitin B is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin B is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression.When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. Interestingly ubiquitin also becomes covalently bonded to many types of pathological inclusions which appear to be resistant to normal degradation.

Publications

Species	Application	Title
human		Cancer Lett; Exosome-mediated transfer of SNHG7 enhances docetaxel resistance in lung adenocarcinoma; Authors - Kai Zhang
	WB	Cell Death Differ; Parkin maintains mitochondrial levels of the protective Parkinson's disease-related enzyme 17-β hydroxysteroid dehydrogenase type 10.; Authors - G Bertolin
	WB	Carcinogenesis; p62/SQSTM1 interacts with vimentin to enhance breast cancer metastasis.; Authors - Si-Si Li
		BMC Res Notes; A monoclonal antibody raised against bacterially expressed MPV17 sequences shows peroxisomal, endosomal and lysosomal localisation in U2OS cells.; Authors - Hans Weiher
human	WB	Autophagy; Sensitive ELISA-based detection method for the mitophagy marker p-S65-Ub in human cells, autopsy brain, and blood samples; Authors - Jens O. Watzlawik

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