Anticorps Monoclonal anti-VWF

VWF Monoclonal Antibody for IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(17)

CloneNo.

3F9F3

Avis (1)

N° de cat : 66682-1-Ig

Synonymes

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 3F9F3

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Galerie de données de validation

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  IHC staining of human breast cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66682-1-Ig (VWF antibody) at dilution of 1:2000 (under 10x lens). Proteolytic pre-treatment mediated antigen retrieval with .

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  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66682-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66682-1-Ig (vwf antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66682-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66682-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using VWF antibody (66682-1-Ig, Clone: 3F9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 4 publications below
• IF: See 14 publications below

Informations sur le produit

66682-1-Ig cible VWF dans les applications de IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: VWF Protéine recombinante Ag25578
• Nom complet: von Willebrand factor
• Symbole du gène: VWF
• Identification du gène (NCBI): 7450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).

Publications

Species	Application	Title
mouse	IHC	J Cell Mol Med; Impact of chronic intermittent hypoxia on the long non-coding RNA and mRNA expression profiles in myocardial infarction.; Authors - Chaowei Hu
rat	IF	Int J Stem Cells; Integrin α4 Positive Subpopulation in Adipose Derived Stem Cells Effectively Reduces Infarct Size through Enhanced Engraftment into Myocardial Infarction; Authors - Zihui Yuan
	IF	Exp Ther Med; Biological activity of a vascular endothelial cell-hydroxyapatite orbital implant complex: An experimental study.; Authors - Weiqi Wu
mouse	IF	Cell Death Dis; Association of N6-methyladenine DNA with plaque progression in atherosclerosis via myocardial infarction-associated transcripts.; Authors - Lianpin Wu
mouse	IF	Inflammation; Low-Shear Stress Promotes Atherosclerosis via Inducing Endothelial Cell Pyroptosis Mediated by IKKε/STAT1/NLRP3 Pathway; Authors - Yifei Lv
rat,mouse	IF	Int J Biol Sci; Metallothionein 3 Potentiates Pulmonary Artery Smooth Muscle Cell Proliferation by Promoting Zinc-MTF1-ATG5 Axis-mediated Autophagosome Formation; Authors - Tianxin Xiong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (01-05-2024)

This monoclonal antibody worked well for immunohistochemistry on human heart sections to stain blood vessels.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Heart

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