CoraLite®647-conjugated CD107a Mouse McAb

CD107a Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite®647 Fluorescent Dye

CloneNo.

H4A3

Cat no : CL647-65051

Synonyms

CD107a, LAMP 1, LAMP1, LAMPA, LGP120



Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 65051-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65051 targets CD107a in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 1
GenBank accession numberBC006345
Gene symbol LAMP1
Gene ID (NCBI) 3916
Conjugate CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/665 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
IF protocol for CL647-conjugated CD107a antibody CL647-65051Download protocol
FC protocol for CL647-conjugated CD107a antibody CL647-65051Download protocol
Standard Protocols
Click here to view our Standard Protocols