CoraLite® Plus 647 Anti-Mouse CD279 (PD-1) (RMP1-30)

CD279 (PD-1) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RMP1-30

Cat no : CL647-65142

Synonyms

CD279, Ly101, PD 1, PD-1, Pdc1, Pdcd1, programmed cell death 1

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Validation Data Gallery

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  FC experiment of BALB/c mouse splenocytes using CL647-65142

  1X10^6 Con-A stimulated BALB/c mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD279 (PD-1) (CL647-65142, Clone: RMP1-30) or isotype control antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: Con-A stimulated BALB/c mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65142 targets CD279 (PD-1) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse PD-1 transfected BHK cells
• Full Name: programmed cell death 1
• GenBank accession number: BC119179
• Gene symbol: Pdcd1
• Gene ID (NCBI): 18566
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 665 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).